ONCOLOGY

ONCOLOGY CHEMICAL LIBRARIES AND BIOLOGICAL ASSAYS

Oncology-Related Chemical Libraries constitute one of the most important directions of development at ChemDiv. Carefully designed focused or target-biased libraries are comprehensive and cost effective approaches toward development of existing programs and design of new programs in this area. Multiple design tools and approaches have been implemented in order to create the library sets. Starting with a broad analysis of the existing oncology “targetscape” and following with various chemoinformatic tools for selection of specific libraries (ligand hopping, flexible docking, pharmacophore search, etc.), ChemDiv is offering more than 30 different chemical libraries with a variety of new scaffolds to choose from.

Biological assays in vitro and in vivo models offer a rapid way to evaluate a new chemical entity. ChemDiv is frequently developing new assays and animals models relevant to oncology and examples are listed below:

Some of the Libraries Include:
  • Modulators of proliferation and differentiators of stem cells
  • HSP90
  • Akt Kinase library
  • Apoptosis library
  • Signaling and developmental pathway modulators
  • Aurora A/B Kinase library
  • BCL2/MCL1 library
  • c-Met Kinase library
  • Chemokine library
  • EphB4 inhibitors library
  • GSK3b library
  • HDAC library
  • IGF-1R library
  • Methyltransferase inhibitors library
  • MK2 inhibitors library
  • p2x7 focused library
  • PDZ library
  • Phosphatases library
  • pI3 Kinase library
  • RAR library
  • Secretase library
  • Serine/Cysteine Proteases inhibitors library
  • Steroids/steroid-like library
  • Sulfotransferase inhibitors library
  • GPCR platform set
  • Ion Channels platform set
  • Kinases platform set
  • Peptidomimetics
  • Natural-like

 

ONCOLOGY-RELATED ASSAYS INCLUDE:

  • Proliferation inhibition assays (Alamar Blue test, BrdU assay)
  • Calcium mobilization assays (FLIPR-based technology for GPCR`s agonists/antagonists, particularly for CXCR4 receptor)
  • Kinase inhibitor assays (ADP Hunter, ELISA)
  • Cell-based assay in C3H10T1/2 cells (Hedgehog inhibition is detected by increase in intracellular Alkaline Phosphatase activity)
  • Cell cycle analysis, apoptosis, in vitro chemoinvasion

Please contact our team at [email protected] to discuss your specific needs and application.

ONCOLOGY IN VIVO

ChemDiv offers research in both human xenografts and syngenic tumors in mice. Xenografts can be grown in nude mice or SCID and primary tumor growth or metastasis can be evaluated. Syngenic tumors can be grown in most mice species. We offer traditional subcutaneous models as well as orthoic and other models and can customize models to meet your needs. Other models can be procured on short notice.

Studies

  • Culture and inoculation of tumor cell lines
  • Monitoring and measurement of tumor growth
  • Administration of therapeutic or preventative dosing protocols
  • Excision and collection of tumors
  • Necropsy with observation for sites
  • “Tumor Growth” – initial studies to determine tumor growth rate in nude mice
  • “Tumor Production” – production of tumor-bearing mice for collection of tumors or performance of therapeutic studies