Cytokines
ChemDiv proposes high throughput screening (HTS) service
Cytokines research platform
- 92 assays, 9 classes of cytokines are available
Interleukins, Interferons, TNF cytokines, TGF-β cytokines, Hematopoietic Cytokines, C Chemokines, CC Chemokines, CXC Chemokines and CX3C Chemokines
- Multiplex and ELISA cell-based assays
- Track record of 25+ years’ experience, expertise in all major therapeutic areas
- Full discovery support of your projects
CADD, synthetic and medicinal chemistry, preclinical biology, animal studies, CMC and formulation
- Screening of up to 1.6M compounds
Cherry-pickable collection
- Support for compound selection
Custom selection and screening set preparation from 1.6M stock available compounds
More details on prices, time, or research specificity - please contact chemdiv@chemdiv.com
Cytokines, being a part of the immune system, are potential targets for drug development to treat cancer, autoimmune diseases, inflammatory diseases, etc. Antagonists of IL-1 (such as IL-1ra) inhibit both IL-1-associated tumor development and IL-1-associated autoimmune diseases development. There are also several examples of successful chemokine targeting: CXCR4 inhibitor, plerixafor, mobilizes hematopoietic stem cells, and CCR5 antagonist maraviroc is used for HIV treatment.
For cytokines studies ChemDiv offers a Multiplex cell-based assay. Key advantages of Multiplex are:
- Magnetic microspheres of designated colors are covered with antibodies of defined binding specificities
- Target cytokine proteins bind to the “capture” antibodies on the surface of the microspheres
- Incubation with “detection” antibodies and reporter molecules allows to measure fluorescence and adsorption
- Microspheres are classified one by one in a flow cell via laser detection system
- Multiplex cytokine assay technology allows to measure numerous analytes in a single sample
Figure 1. Multiplex assay principle
For the following lead characterization during the inhibitor development ELISA cell-based assays are used. The key points for ELISA are:
- Numerous cytokines produced by cell serve as antigens for the studies
- The antigens are bound to the wells of a microplate
- Specific to the antigen antibodies, then reporter antibodies and coloring agents are added to tag the molecule of interest
- Change in color allows to indirectly measure the amount of cytokines
List of targets: