Epitranscriptome Focused Small Molecule Library
DescriptionLibrary of Epitranscriptome Focused Small Molecule Library contains 21,000 compounds.
Within the field of molecular biology, the epitranscriptome includes all the biochemical modifications of the RNA (the transcriptome) within a cell. Epitranscriptomics involves all functionally relevant changes to the transcriptome that do not involve a change in the ribonucleotide sequence. Thus, the epitranscriptome can be defined as the ensemble of such functionally relevant changes.
• A variety of abundant RNA modifications have been shown playing a critical role in gene regulation.
• “Writers”, “readers”, and “erasers” : RNA-binding proteins that perform post-transcriptional modifications and support cellular processes vital for RNA homeostasis.
• Aberrant RNA modifications are therapeutically relevant for the immune system and the central nervous system:
➢ Prompt the growth of acute myeloid leukemia cells.
➢ Associated with various neurodevelopmental and neuronal disorders – autism, epilepsy, brain development abnormalities, obesity, microcephaly, Prader-Willi syndrome, fragile X syndrome, major depressive disorder, etc.
➢ A regulatory mechanism of brain development and function.
Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons. Kate D. Meyer, Yogesh Saletore, […] Cell 149, 1635–1646 (2012) doi:10.1016/j.cell.2012.05.003
Above the Epitranscriptome: RNA Modifications and Stem Cell Identity. Francesco Morena, Chiara Argentati, […] Genes 9(7):329 (2018) doi: 10.3390/genes9070329
Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells. Dali Han, Jun Liu, […] Nature 566, 270–274 (2019) doi:10.1038/s41586-019-0916-x
Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Jeffrey J. Ishizuka, Robert T. Manguso, […] Nature 565, 43–48 (2019) doi:10.1038/s41586-018-0768-9
Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control. Isaia Barbieri, Konstantinos Tzelepis, […] Nature 552, 126–131 (2017) doi:10.1038/nature24678
m6A Demethylase ALKBH5 Maintains Tumorigenicity of Glioblastoma Stem-like Cells by Sustaining FOXM1 Expression and Cell Proliferation Program. Zhang S, Zhao BS, […] Cancer Cell. 31(4):591-606.e6 (2017) doi: 10.1016/j.ccell.2017.02.013
The N6-methyladenosine (m6A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells. Ly P Vu, Brian F Pickering, […] Nature Medicine 23, 1369–1376 (2017) doi:10.1038/nm.4416