Growth factor receptors
Growth factor receptors
ChemDiv proposes high throughput screening (HTS) service
Growth factor receptors research platform
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92 assays, 23 classes of GFRs are available
Eph receptors, FGF receptors, IGF receptors, TGFβ receptors, Wnt receptors, VEGF receptors, RET receptors and other
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2 lead characterization methods: flow cytometry and western blotting
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Track record of 25+ years’ experience, expertise in all major therapeutic areas
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Full discovery support of your projects
CADD, synthetic and medicinal chemistry, preclinical biology, animal studies, CMC and formulation
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Screening of up to 1.6M compounds
Cherry-pickable collection
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Support for compound selection
Custom selection and screening set preparation from 1.6M stock available compounds
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The best choice of stock screening libraries towards GFRs
4 GFR-focused libraries are available:
Inhibitors of beta-Catenin Signaling Library
Angiogenesis library
C-Met Library
Developmental Pathway (Hh/Wnt) Set
More details on prices, time, or research specificity - please contact chemdiv@chemdiv.com
Growth factor receptors (GFRs) malfunction leads to the upregulated cell division and overpopulation,which may result in cancer, cardiovascular diseases (CVD) or neurodegenerative disorders initiation and progression. The importance of VEGF receptor inhibition comes from its engagement in tumor progression and survival. IGF receptor downregulation, in turn, sensitizes cells to antitumor treatments. Apart from cancer studies, EGFR inhibitors, such as gefitinib, are explored as a therapeutic strategy for CVD.
Studies of growth factor receptors heavily rely on cell-based assays used in the process. GFRs can be studied via the following assay platforms:
Flow cytometry
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A suspended sample of dye-loaded cells is arranged in a single line to pass through laser beam one by one
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Signal from passing cells is registered to count and characterize them
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GFRs activity is measured by the decrease in fluorescent signal caused by the dilution of proliferation dye in daughter cells (Figure 1)
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Approximately 10000 cells can be analyzed in less than one minute
Figure 1. The algorithm for cell proliferation measurement using flow cytometry proliferation dye assay. Flow cytometry analysis allows to separate the peaks from different generations of cells based on the fluorescent signal.
Western blotting
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Proteins are separated via the gel electrophoresis based on their molecular weight
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Polyvinylidene fluoride (PDVF) membrane produces a band for each of the proteins
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Incubation with specific antibodies leads to the localization of the protein of interest
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The thickness of the remaining band directly depends on the amount of protein present
Targets
Growth factor receptors are classified based on their ligand (growth factor):