Inflammation
ChemDiv proposes high throughput screening (HTS) service
Inflammation research platform
- 42 assays, 5 groups of proteins involved in the inflammatory processes are available
Nod-like receptors, Toll-like receptors, Inflammatory cytokines, G-protein coupled receptors and other
- High-content HTS platform
- ELISA and flow cytometry assays for lead characterization
- Track record of 25+ years’ experience, expertise in all major therapeutic areas
- Full discovery support of your projects
CADD, synthetic and medicinal chemistry, preclinical biology, animal studies, CMC and formulation
- Screening of up to 1.6M compounds
Cherry-pickable collection
- Support for compound selection
Custom selection and screening set preparation from 1.6M stock available compounds
- The best choice of stock screening libraries towards inflammation
6 inflammation-related libraries are available:
TLR-8 ligands Library
ACE2 Library
Chemokine Receptor-Targeted Library
Serine Proteases Inhibitors Library
Macl-GPIb alpha Interaction Library
Glucocorticoid receptors Library
Steroid-like Compounds
More details on prices, time, or research specificity - please contact chemdiv@chemdiv.com
Inflammatory proteins’ targeting is highly specific because of the diversity of the inflammation signaling pathways. Nowadays the most established antiinflammatory drugs are corticosteroids; however, their use is limited due to the variety of responses between patients. The other approaches to the inflammation therapy exists, such as TLR4 inhibition, targeting the “top” of the inflammatory pathway and therefore reducing variability of possible outcomes (Figure 1).
Figure 1. Toll-like receptor 4 (TLR4) regulation of the inflammation development. From “TLR4 (Toll-Like Receptor 4) Mediates the Development of Intracranial Aneurysm Rupture” by Kazuha Mitsui et al.
High-throughput screening is an effective way to study the influence of compounds on the inflammatory processes. ChemDiv offers following cell-based assay platforms:
High content (HC)
- The cellular model is exposed to the stimulus of interest for inflammatory response
- The added fluorescent compounds interact with specific targets
- Magnitude of the fluorescence is processed and analyzed by complex algorithms automatically
- The signal from the images of cells is recorded from all the wells of 384-well microplate
ELISA
- Numerous proteins taking part in inflammation pathways serve as antigens for the studies
- The antigens are bound to the wells of a microplate
- Specific to the antigen antibodies, then reporter antibodies and coloring agents are added to tag the molecule of interest
- Change in color allows to indirectly measure the inflammatory processes activity
Flow cytometry
- A suspended sample of dye-loaded cells is arranged in a single line to pass through laser beam one by one
- Signal from passing cells is registered to count and characterize them
- Overexpressed inflammatory markers are tagged by fluorescently labeled antibody
- Approximately 10000 cells can be analyzed in less than one minute
List of targets: