Proteases

Proteases

ChemDiv offers high throughput screening (HTS) service
Proteases research platform

• 56 protein assays depending on groups available

• pNA assays

• 7 protease-focused (Protease Library, Serine Proteases Inhibitors Library, Cysteine Proteases Inhibitors Library, Human Proteases Annotated Library, 3CLpro Library, Proteases Library, PLpro Library), 106K compounds in total

• Our support in compound selection based on your research project

• Screening from 10000 compound sets up to 300000 and more

• A quick start of your research project

• 25 years of experience

Viral proteases are common targets for drugs: nelfinavir inhibits HIV-1 protease, nirmatrelvir inhibits 3C-like protease of coronaviruses, telaprevir inhibits NS3/4A serine protease of the hepatitis C virus. Proteases of eukaryotic cells may also be targeted: matrix metalloproteinase inhibitors, like tanomastat, can be used as antimetastatic agents. Additionally, neutrophil-expressed elastate, when combined with its activator, eosinophil cationic protein, showed antitumor activity, although that activity was lower in vivo, which is thought to be due to the abundance of protease inhibitors in the tumor environment.

CD95 and H1.0 assay after ELANE administration, from ‘Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis’ by Cui et al.

We use pNA assays to monitor protease activity. pNA can be characterised as:

• pNA peptides are a protease substrate
• Protease cleavage and hydrolysis of the pNA peptides releases p-nitroaniline
• Free p-nitroaniline is yellow
• Color intensity is then measured

ChemDiv provides many stellar protease assay options, with the ones more frequently asked for written in the list below:

• ACE, ACE2
• ADAM (10, 17)
• BACE1
• CASP (1-10, 14)
• CPN1
• CTS (B-V)
• DPP (4, 8, 9)
• F (2, 7, 9-12)
• GZMB
• KLK (1-2, 5, 7, 12-14), KLKB1
• MME
• MMP (1-14)
• PLAU
• PROC
• PRSS1
• TMPRSS11D, TMPRSS2
• TPSG1