Protein-protein interactions
ChemDiv offers high throughput screening (HTS) Service protein-protein interactions (PPI) research platform.
- Over 50 assays
- Over 200 000 compounds in PPI focused library
- Custom-developed assays
- FRET, TR-FRET and fluorescence polarization-based HTS assay platforms
- Six libraries:: Bcl2-PPI Inhibitors Library, PDZ PPI Library, 3D Mimetics PPI Library, Protein-Protein Interaction Library, Nonpeptide Peptidomimetics PPI Library, PPI Inhibitors Tripeptide Mimetics, 269K compounds in total
- Broad expertise in PPI assay development, optimisation and validation
- A quick start of your research project
- Protein’s ability to perform complex functions together makes protein-protein interactions an area of intense research.
For more details on prices, time, or research specificity - please contact chemdiv@chemdiv.com
Chemdiv offers FRET, TR-FRET (Tb or Eu) and fluorescence polarization assays. TR-FRET can be summarized in these four points:
- There is a transfer of energy between proximate donor and acceptor fluorophores
- Each interacting protein is tagged with a antibody+fluorophore
- Formation of a complex brings fluorophores close enough for energy transfer
- Acceptor emissions are measured
Fluorescence polarization assays:
- Fluoroscent dye is excited by polarized light
- Degree of polarization of reemitted light is inversely proportional to rotation and random movements
- A large complex has limited rotation, so the degree of polarization remains high
- Degree of light depolarization is then measured
We at Chemdiv have a wealth of experience in PPI assay development, optimisation and validation, so we will be happy to tackle any desired targets. Please inquire for further details.