Kinases
Kinases
ChemDiv offers high throughput screening (HTS) service
Kinases research platform
- 377 protein assays depending on groups available (separated by alphabet order for your convenience)
- 4 HTS platforms: ADP Hunter, Z’-lyte, LANTHAScreen, LANCE.
- The best choice of screening libraries on the market: 11 kinase-focused libraries to choose from (Aurora A-B Kinases Targeted Library, Human Kinases Annotated Library, Proline Kinase Library, Allosteric Inhibitors, Protein Kinases Inhibitors Library, C-Met Library, Akt-Targeted Library, Type II Kinase Inhibitors Library, Kinases Library, SH2 PTB Focused Library, DGK Inhibitors Library), 145K compounds in total
- Our support in compound selection based on your research project
- Screening from 10000 compound sets up to 300000 and more
- A quick start of your research project
- 25 years of experience
More details on prices, time, or research specificity - please contact chemdiv@chemdiv.com
Mutations in kinases, both inherited and somatic, are linked to numerous diseases, cancer in particular: ALK mutations – neuroblastoma, BTK – lung carcinoma, JAK family – immunodeficiency, PINK1 – early-onset Parkinson. Kinase inhibitors are a mainstay of cancer therapy: trametinib MEK1/MEK2 inhibitor, dabrafenib is a BRAF inhibitor, to just list a few. We would be remiss to not note, however, that kinase inhibitors are also used in the fields of autoimmune diseases and for immunosuppression – rapamycin is the most well-known example.
We utilize a variety of kinase assay methods, including ADP Hunter, Z’-lyte, LANTHAScreen and LANCE. ADP Hunter highlights:
- Kinases use ATP as a substrate and leave ADP as a byproduct
- ADP is then combined with other reagents to produce a fluorophore
- Fluorophore gives a measurable signal
- Stronger signals correspond to higher levels of activity
The second platform, Z’-lyte:
- Threonine/tyrosine/serine in FRET-peptide accepts a phosphate
- Protease cleaves residues without a phosphate
- Protected peptides continue their FRET, while cleaved ones do not
- A donor/acceptor emission ratio serves as the resulting measurement
LANTHAScreen:
- Kinases phosphorylate the substrate,
- EDTA stops kinases
- Tb-antibodies bind phosphorylated substrate, resulting in TR-FRET
- Higher TR-FRET corresponds to higher kinase activity
LANCE:
- Kinases phosphorylate a special substrate
- EDTA stops kinases
- Eu-antibodies bind phosphorylated peptides, resulting in light emission
- Light serves as a measure of kinase activity
Kinase assay methods
As for the targets themselves, ChemDiv provides assays for a large variety of kinases. You can add kinase assays for all platforms mentioned to your shopping cart from the list below: